National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Evaluation of the diagnostic utility of PCR in comparison to the conventional test. Descriptive study. All patients with suspected microbial keratitis presenting between October 2012 to June 2014 at the Ophthalmology OPD, JIPMER hospital. A total of 50 consecutive non-duplicated cases of keratitis were included in the study period of two years. All the samples were subjected to the conventional test like microscopy using Gram stain and modified trichrome stain, and PCR for Acanthamoeba and Microsporidia. Microscopy, PCR, age and sex distribution of patients with keratitis, risk factors, clinical presentation of patients. Mean age group of the patients in this study was 48.3 years (range 10-90) and majority of them were females (54%). The predominant symptom with which the patients presented in our study was pain (60%).Corneal trauma with vegetative matter was a major risk factor accounting for 20%. Out of the 50 samples, one (2%) of the specimens was positive for Acanthamoeba and two (4%) were positive for microsporidia by PCR, while, none of the specimens was positive by microscopy for Acanthamoeba and Microsporidia on Modified trichrome stained smears. Hence, this establishes the fact that PCR is superior to microscopy as it is a sensitive cum rapid method for the diagnosis of keratitis due to Acanthamoeba and Microsporidia.
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