International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 9, Issue:7, July, 2020

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(7): 189-196

Comparative Evaluation of Rapid Crossing Techniques for Wheat x Maize System of Doubled Haploid Production
Gagandeep Singh, Puja Srivastava and G. S. Mavi*
Wheat section, Department of Plant breeding & Genetics, PAU, Ludhiana, India
*Corresponding author

Five different emasculation methods viz. standard technique for wheat x maize crosses, early pollinations on non-emasculated spikes, clipped glume facilitated emasculation, plastic bag emasculation and hot water emasculation were compared. Clipped glume facilitated emasculation resulted in lowest caryopses formation frequency. Early pollination on non-emasculated spikes gave reasonably high embryo formation frequency while omitting tedious emasculation steps. An innovative method based on non-dehiscence and functional sterility of anthers exposed to high humidity in plastic bag showed good promise though considerable proportion of selfed seed (32.99%) was observed. The method that proved best involved hot water (430 C for 4 min) treatment of detached tillers. It showed high embryo formation frequency (30.88 %) and low selfed seed set (3.20%). Some level of female sterility observed in this method is needed to be tackled through further refinement.

Keywords: Wheat, maize, Plastic bag, clipped glume

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How to cite this article:

Gagandeep Singh, Puja Srivastava and G. S. Mavi. 2020. Comparative Evaluation of Rapid Crossing Techniques for Wheat x Maize System of Doubled Haploid Production.Int.J.Curr.Microbiol.App.Sci. 9(7): 189-196. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.