National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Fusarium wilt of bitter gourd is caused by Fusarium spp. Ten isolates of Fusarium were isolated from bitter gourd growing areas of Coimbatore district to study the cultural and morphological characters of Fusarium sp. From the survey, it was found that the highest PDI was recorded in Naatukalpalayam 1 with 52.63%. The mycelial diameter of ten isolates was ranged from 6.2 to 9.0 cm at seven days of incubation on PDA. The highest colony diameter of 9.0cm was observed in BF 2 and BF 7 followed by BF5, BF8, BF9, BF4, BF6, BF10 and BF3. Minimum colony diameter was observed in BF1 with 6.1cm. Different media studies have shown that Fusarium solani could grow well in PDA and PSA. All ten isolates have produced micro, macroconidia and chlamydospores. The size (µm) of microconidia ranged from (9.2-11.8) x (3.2-4.6) and macroconidia is (15.7-24.4) x (3.8 – 5.0) and they are hyaline in color. There are 2-4 septations in macroconidia. Macroconidia are sickle shaped with pointed tips and microconidia are elongated oval in shape. Chlamydospores were seen on intercalary, terminal portion of mycelium and oval in shape. PCR amplification with universal primers of ITS1 and ITS4 yielded approximately at 560 bp. Sequencing of ITS regions followed by BLAST search confirmed the associated fungus as Fusarium solani.
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