National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Digested biogas slurry is a rich lignocellulosic substrate, consistent with the idea of sustainable agriculture and development. This work is aimed at utilization of digested biogas slurry as a substrate for the quantitative screening of lignocellulose degrading fungi. In this study, the potential of digested biogas slurry as a substrate to determine the lignocellulolytic enzyme production after 8 days of incubation at 30±2ºC (mesophilic) and 50±2ºC (thermophilic) was investigated. Standard mesophilic culture Phanaerochaete chrysosporium MTCC 787 showed maximum enzyme activities (3.9U/ml endoglucanase, 2.09 U/ml exoglucanase, 29.45 U/ml β-glucosidase, 55 U/ml xylanase, 69.5 U/ml mannanase, 48.33U/ml lignin peroxidase, 13.3 U/ml Manganese peroxidase) followed by isolate A5, A4, A12. Among thermophilic cultures, isolate A31 is the best cellulolytic fungal culture. Thermoascus aurantiacus (1.97U/ml endoglucanase), isolate A25 (23.08U/ml β-glucosidase, 2.35U/ml exoglucanase, 63U/ml Mannanase) and A31 (48.08 U/ml xylanase, 11.5 U/ml lignin peroxidase, 5.6U/ml laccase) are thus the best lignolytic fungal cultures. The results show that digested biogas slurry, which otherwise is considered as a waste of biogas plants, can be efficiently utilized to screen different fungi for their lignocelluloses degrading potential. Mesophilic cultures Phanaerochaete chrysosporium, isolate A4 and A5 and thermophilic cultures Thermoascus aurantiacus, isolate A25 and A31 are the best lignocelluloses degraders.
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