A potential non-Saccharomyces yeast species, identified as Wickerhamomyces anomalus SZ1 strain ( Gupta, et al., 2018), which gave even higher (33%) mannan oligosaccharides (MOS) than that obtained from the traditionally used Saccharomyces cerevisiae strain were selected for optimization of suitable media study for maximum yield of MOS by the one factor at a time (OFAT) method. Mannose was found to the best carbon source for optimum production of MOS, which significantly enhanced the yield by 1.2 folds of MOS at 2% mannose concentration as in place of dextrose in YEPD media. Higher concentration of Mannose cannot significantly (pË‚0.05) enhance the MOS production further. 2% peptone and 1% yeast extract in combination were found to be the best nitrogen source. An initial pH 6.0, temperature 32⁰C and shaking condition at 180 rpm for a period of 96 hours were found significantly favour the MOS production. the result revealing that 5% (1.05x10⁸cfu/mL) is the optimum inoculum size to attain the maximum MOS yield (701.13±23.23 mg/L at 96 hours incubation) that was 2.0 fold higher than that to incubated at 24 hours and 1.2 fold higher to that 1% (2.1x10⁷cfu/mL) inoculum density but economically yield was insignificant with period of 72 (656.67±23.12 mg/L) to 96 (701.13±23.23 mg/L) hours incubation. It was concluded that W. anomalus SZ1 strain can be grown on optimized media up to 72 hours and used as an alternative of S. cerevisiae yeast for commercial mass scale MOS production for human food and animal feed industries in future.

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