Mycorrhizal fungi occur in most of the soils and colonize roots of many plant species. A greenhouse experiment was carried out to study the efficiency of Arbuscular Mycorrhizal fungi indigenous to Arunachal Pradesh in uptaking plant nutrients for the Piper mullesua plantlets at different soil condition i.e., sterilized soil, unsterilized soil and field condition. As the sterilized soil condition is difficult to understand the performance of AM fungi, field experiments are necessary to understand the effect of mycorrhizal fungi on yield of crops in field condition. The experiment was done to determine the effectiveness of mycorrhizal fungal inoculated with P. mullesua plantlets assessing its effect on plant growth and plant nutrition when compared with non-mycorrhizal P. mullesua plantlets in sterilized, unsterilized and field condition. For this ten different mycorrhizal fungal species isolated from various land use systems such as forest area, jhum fields, home gardens as well as natural habitat of piper plants were inoculated with the plantlets of P. mullesua in three different set of experiment. In present study G. claroidium (2.238gm ±0.209), G. aggregatum (2.122gm ±0.057)  and G. versiforme (2.109gm ±0.051) were found more capable in  producing better growth by infecting Piper mullesua plantlets under field soil as compared to unsterilized and sterilized soil condition. Plant phosphatase content was significantly (p>0.001) higher in the seedlings grown in unsterilized soil, followed by in field condition and least in the sterilized soil. These inocula were efficient in establishing beneficial relationship with other native microorganisms of soil.   As chemical fertilizers possess threat to the environment by polluting soil and environment, these efficient mycorrhizal species can work as potential biofertilizer for agriculturally important crops.

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