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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 9, Issue:5, May, 2020

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(5): 2411-2422
DOI: https://doi.org/10.20546/ijcmas.2020.905.275


Morphology and invitro Study of Chemicals, Plant Extracts, Bio-Agents against Pestalotiopsis mangiferae, Causing Grey Leaf Spot of Mango in Manipur, India
Ch. Inao Khaba*, Marjit Chandam, N. Ajitkumar Singh, Ch. Premabati Devi and Bireswar Sinha
Department of Plant Pathology, College of Agriculture, CAU, Lamphelpat-795004, Manipur, India
*Corresponding author
Abstract:

A study was conducted in vitro condition to analyse the morphology and efficacy of bio agent, plant extract and some chemical against P. mangiferae that were collected from different districts of Manipur. The morphological character under study consists of colony and conidial characteristics such as colour, shape, size and appendages. The cultural growth colour of P. mangiferae on PDA differed from concolour to versicolour fuliginous. The conidial shape varied from oval and spherical to elliptical with prominent appendages. The conidial length and width were 22.9 and 5.7 µm respectively. The diseased sample which was collected from different district of Manipur consists of three septation and the number of conidial appendages was found 2-3 numbers. Among seven antagonists namely Penicillium citrinum, Trichoderma atroviride, T. ovalisporum, Hypocrealixii, T. harzianum (69 & 131) and T. asperellum evaluated in vitro, T. asperellum revealed the best in inhibiting the growth of the fungus (85.8%). Among three plant extracts viz. garlic, neem and sweet flag evaluated in vitro, garlic extract (3.0%) showed the best result (100.0%). Among the seven chemicals viz. carbendazim, thiophenate methyl, mancozeb, imidacloprid, fipronil, profenophos and thiomethoxam evaluated in vitro, thiophenate methyl (0.05%) and carbendazim (0.05%) showed the best result with 100.0 per cent inhibition in fungal growth.


Keywords: Morphology, Antagonists, Plant extracts, Chemicals, P. mangiferae, Mango

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How to cite this article:

Ch. Inao Khaba, Marjit Chandam, N. Ajitkumar Singh, Ch. Premabati Devi and Bireswar Sinha. 2020. Morphology and Invitro Study of Chemicals, Plant Extracts, Bio-Agents against Pestalotiopsis mangiferae, Causing Grey Leaf Spot of Mango in Manipur, India.Int.J.Curr.Microbiol.App.Sci. 9(5): 2411-2422. doi: https://doi.org/10.20546/ijcmas.2020.905.275
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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