National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Characterization and quantification of diversity in plant genetic resources were important to identify cultivars and clones, decipher relationships including parentages, and to efficiently manage germplasm collections. The present studies was undertaken to find out genetic diversity among twenty-one genotypes of rose (Rosa species) using EST-SSR markers. Out of the total 35 EST-SSR primers screened, a set of 12 primers showed polymorphism. PCR amplification yielded a total of 31 amplified products with a range 2 and 4 bands per primer (amplified size 90bp -550bp). The polymorphic information content (PIC)value of the primers ranged from (0.22- 0.96) with Highest PIC and Marker Index (MI) was recorded by primers RS-18 followed by Primer RS-30 and RS-10. Hence, primer RS-18 and Primer RS-30 were found to be highly informative. Jaccard’s similarity coefficient ranged from 0.143 to 0.923. Minimum similarity was observed between the genotype (Lady X) and (Happiness) (0.143). The similarity coefficient used to construct a dendrogram using UPGMA cluster analysis. The dendrogram grouped the twenty-one rose genotypes into three distinct clusters. Therefore, from this study the genotypes (Lady X) and (Happiness) (0.143) possesses diverse genetic background which can also be used as parent for further hybridization programme.
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