The water samples of different sugar producing stages from eight selected sugar factories were collected to explore the possibility of its utilization as fresh water. Samples were collected from Fresh / Raw water; 1^st body condensate; 2^nd body condensate; 3^rd body condensate; 4^th body condensate; Pan condensate; UGR Cold; UGR Hot; ETP Inlet and ETP Outlet. On an average the macroscopic characters exhibited off white colour, round shape, flat elevation and rough/slimy appearance in all 8 sugar factories whereas microscopic characters of these water samples showed rod /cocci shape and both gram positive and grams negative bacteria in all eight  selected sugar factories except vapour condensates exhibited negligible growth of the same. This indicates that vapour condensates are most promising and can be converted into fresh water after required processing. Various Bacterial strains were identified viz E.coli, B.subtilis, P.putida, Salmonella spp, S.aureus and B.cereus etc. It was observed that fungal colony was only seen in water sample of fresh water and in ETP inlet and outlet. Maximum numbers of fungal species identified were Alternaria gaisen, Aspergillus flavus, Aspergillus candidus, Aspergillus niger, Aspergillus nidulans, Peniciilium pinophillum and Trichoderma viride, this database created a novel record of the fungal diversity associated with sugarcane industrial effluent

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