National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The microorganisms most commonly used as probiotics are lactic acid bacteria, especially those of the genus Lactobacillus. In the present study, Lactobacillus acidophilus were isolated from two different pooled samples homemade curd and commercial curd, were characterized for their antiproliferative activity. The antiproliferative effects of the strains were investigated using the MTT assay with liver cancer (Hep G2) cell line. The results showed that the Lactobacillus strains had good antiproliferative effects in liver cancer cell line. Further the viability of cells was observed with the help of fluorescent microscopy by dual staining technique. Lactobacillus acidophilus can be considered as potential probiotic bacteria for humans because of their antiproliferation effect in cancer cells. In this study, effect of the samples on expression of Bax and Bcl2 gene was analysed by RT -PCR. β-Actin was chosen as an internal control to normalize the gene expression. The study indicate that the MTT assay for the strains of Lactobacilus acidophilus isolated from the sample has cytotoxicity effect. Based on the IC50 value, homemade curd showed better percent of inhibition than commercial curd and Bcl2 gene showed fold up regulation when compared to Bax gene.
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