National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
In the present study ten different Bacillus isolates were isolated from various soil samples and their identification was performed on the basis of Gram staining, colony morphology and biochemical tests. Gram positive rod shaped bacteria showing large, circular, opaque colonies having white or greyish white appearance were preserved on Nutrient agar slants for further identification. All isolates showed positive results for Catalase, Urease, Oxidase and motility biochemical tests. For other biochemical tests such as Citrate utilization, Vogues Proskauer, Nitrate reduction, Triple sugar iron Agar test, mixed results were obtained. Phosphate solubilisation and Potassium solubilisation activities of different isolates was performed. Out of ten isolates, none of the isolates solubilised phosphate on Pikovskaya’s agar medium at 30°C after 15 days of incubation. Potassium solubilising activity was shown by two isolates (Sample code 3 and 9) out of ten. Among these two isolates, Sample code 3 showed highest potassium solubilisation and the clear zone on Aleksandrov agar medium was 9 mm and that of Sample Code 9 was 5 mm, after 10 days of incubation at 30°C. Further, the isolates were screened for the production of amylase and its assay has been performed. Seven isolates displayed zone of clearance in starch hydrolysis test except Sample code 5, 7 and 8. Sample code 5, 7 and 8 isolates gave negative results for TSI test also. The isolates were subjected to amylase activity test. Maximum amylase was produced by Sample code 9 at 3.90 mg/ml after 24 hours of incubation. Sample code 10 showed least amylase production at 0.86 mg/ml.
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