National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The fowl adenoviruses have been studied extensively because of their involvement in many pathogenic conditions of poultry and considerable interest to use as gene delivery vehicles. The major structural proteins of virion include hexons and pentons (penton base and fiber). Among these proteins, only hexons have been characterized extensively at molecular level. The present study was conducted for molecular characterization of penton base gene. Liver homogenates of nine fowl adenovirus 4 (FAdV 4) field isolates were propagated in chicken embryo liver cell culture and total genomic DNA was extracted. The PCR amplification revealed a 1675 bp product. Further cloning was done in pGEM-T vectors and subjected to sequencing. The nucleotide sequences were compared with other sequences of NCBI database. The sequence homology within FAdV 4 isolate sequences was 99% to 100%, with FAdV10 99% , with FAdV A 67% and FAdV D 69-70 %. Phenogram analysis of nucleotide sequences showed close association between FAdV 4 and FAdV 10 but amino acid sequences revealed differences and recombinations were evident. Primary structural analysis revealed highly fragile nature of penton base protein. Secondary structure analysis revealed high concentration of proline residues at N-terminal ends signifying its role in virus-host interactions and bonding with other structural proteins.
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