National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Cervical cancer prevalence is increasing every year. The major cause of this cervical cancer is the infection of high-risk Human papillomavirus (HPV) types. HPV variation is related to its ethnicity and geographical source. The objective of this study is to analyze HPV genetic diversity from the two Sumatran regions, Pekanbaru and Padang. Twenty DNA samples from cervical cancer patients were used in this study. HPV detection was carried out using MY09/11 primer. Positive HPV samples were amplified with OPO-6, OPO-18 and OPO-19 primer, and the dendrogram was constructed with UPGMA (Unweighted Pair Group Method with Arithmetic Mean) method. HPV was detected in fourteen DNA samples. PCR amplification by OPO primer showed multiple bands. The dendrogram results of the three primers, DNA samples divided into two groups X and Y. Based on the dendrogram, HPV classified on genotypes and source origin. RAPD data describe HPV variance, but still need further analysis for comprehensive HPV diversity.
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