International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 9, Issue:3, March, 2020

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(3): 3240-3251

Effective Surface Sterilization Method using Plant Preservatives Mixture and Shoot Multiplication of Clinacanthus nutans
Florisa Landa, Yvonne C. Chia, Roslin Ombokou and Zaleha A. Aziz*
Faculty of Science and Natural Resources, University Malaysia Sabah, Jalan UMS, 88400 Kota Kinabalu, Sabah, Malaysia
*Corresponding author

Effective surface sterilization and shoot regeneration protocols for Clinacanthus nutans are described.  Nodal segments of C. nutans were sterilised by single- and double-stage sterilisation methods. In the double-stage method, explants were soaked in Plant Preservative Mixture (PPM) at various concentrations (0, 1.25, 2.50, 5.0 µL/mL). For direct shoots regeneration, MS media were supplemented with TDZ, BAP, or KN in single or in combination (BAP+NAA or TDZ+NAA). For indirect shoot regeneration, single application (BAP or KN) and combination treatment (TDZ+BAP or TDZ+KN) of cytokinins were evaluated. The use of PPM (1.25 µL/mL) resulted in survival rate of explants at 82 ± 4.5 %. Direct shoot multiplication was the highest on the medium with 1.0 mg/L BAP + 0.1 mg/L TDZ where explants produced 20 ± 1.5 % primary, 57 ± 6.2 % secondary and 77 ± 6.6 % tertiary shoots respectively. The medium supplemented with BAP at 0.5 mg/L induced the highest number of shoots indirectly (3.5 ± 0.1 shoots/explant) while BAP at 1.5 mg/L induced the highest percentage of explant forming callus (84.4 ± 5.1 %). Therefore, double sterilisation method using Clorox and PPM at 1.25 µl/ml was optimum for surface sterilisation. A protocol for establishment of C. nutans culture is established.

Keywords: Clinacanthus nutans, Plant Preservative Mixture, Surface sterilization and shoot regeneration

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How to cite this article:

Florisa Landa, Yvonne  C. Chia, Roslin Ombokou and Zaleha A. Aziz. 2020. Effective Surface Sterilization Method using Plant Preservatives Mixture and Shoot Multiplication of Clinacanthus nutans.Int.J.Curr.Microbiol.App.Sci. 9(3): 3240-3251. doi:
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