National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present study was carried out on 3-4 weeks old pathogen free broiler chicks (6 in numbers), maintained on standard feed from Ranchi Veterinary College (BAU) poultry farm . During the total lymphocyte count, lymphocytes appeared very distinctly from monocytes obtained from white buffy coat of mononuclear cells after centrifugation with Ficoll-paque by trypan blue dye exclusion technique with the help of haemocytometer. T-lymphocytes/T-cells showed characteristic greenish yellow fluorescence when stained with FITC (1:10 dilution) tagged antiserum raised against thymocytes. The mean of total lymphocytes & T-lymphocytes of 6 chicks taken as the blood values for time interval of one week on all days of examination were found to be highly significant (p<0.01). T-lymphocytes were found to be about 70% of total lymphocytes in healthy chicks in all days of examination persistently; although their number (total lymphocytes and total T-lymphocytes) increases with age.
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