International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 9, Issue:3, March, 2020

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(3): 2484-2489

Status of Total Lymphocytes and T-Lymphocytes Population in Healthy Broiler Chicks
Kalpana Suman1* and Arun Prasad2
1Department of Biotechnology, Sai Mahavidyalaya (Bhilai), Pt.Ravishankar Shukla University, Raipur (Chhattisgarh) India
2Department of Veterinary Microbiology, Ranchi Veterinary College, Kanke, Ranchi (Jharkhand), India
*Corresponding author

The present study was carried out on 3-4 weeks old pathogen free broiler chicks (6 in numbers), maintained on standard feed from Ranchi Veterinary College (BAU) poultry farm . During the total lymphocyte count, lymphocytes appeared very distinctly from monocytes obtained from white buffy coat of mononuclear cells after centrifugation with Ficoll-paque by trypan blue dye exclusion technique with the help of haemocytometer. T-lymphocytes/T-cells showed characteristic greenish yellow fluorescence when stained with FITC (1:10 dilution) tagged antiserum raised against thymocytes. The mean of total lymphocytes & T-lymphocytes of 6 chicks taken as the blood values for time interval of one week on all days of examination were found to be highly significant (p<0.01). T-lymphocytes were found to be about 70% of total lymphocytes in healthy chicks in all days of examination persistently; although their number (total lymphocytes and total T-lymphocytes) increases with age.

Keywords: Total lymphocyte, T-lymphocyte, Immune system, Broiler chicks.

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How to cite this article:

Kalpana Suman and Arun Prasad. 2020. Status of Total Lymphocytes and T-Lymphocytes Population in Healthy Broiler Chicks.Int.J.Curr.Microbiol.App.Sci. 9(3): 2484-2489. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.