National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present study was conducted to characterize Tomato Leaf Curl Virus infecting tomato in south Gujarat region. Infected leaves showed typical leaf curl symptom with upward/downward curling along with vein clearing of the leaves. Using PCR a part of DNA-A molecule of ~1200 bp was amplified with a Begomovirus specific primers confirming it to be Begomovirus. On sequencing, a 1125 bp nucleotide sequence (Accession no- KU921251) was obtained. Amplified fragment had two genes viz., virus coat protein (V1) gene and pre coat protein (V2) gene. The sequenced virus showed highest identity (94%) with Tomato leaf curl Gujarat virus [Nepal] segment DNA A, [AY234383.1] followed by 93% sequence identity with thirteen different tomato Begomoviruses which were the strains/isolates of Tomato leaf curl Gujarat virus or Tomato leaf curl New Delhi virus [DQ629101.2]. The virus was named as Tomato leaf curl Gujarat virus [Nepal] [India:Nvs:Tom:2016] and was abbreviated as ToLCGV [Nepal] [India:Nvs:Tom:2016]. Phylogenetic analysis of the sequenced virus with the other Begomoviruses of the different crops from the different location indicated that the virus was typically a leaf curl virus.
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