National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
To study the effect of substrate concentration on soil enzyme Urease in selected soils. Forty soil samples were assayed to measure the activity of the soil enzyme Urease among them four soil samples two Alfisols and two Vertisolssoils with high activity were selected for further study. Urease activities of the surface soils expressed as µg of NH4+ released g-1 soil h-1 ranged from 5.9 to 16.0 with an average value of 8.74.Soil enzyme Urease activity increased with an increase in substrate concentration in the beginning and almost reached a plateau at a substrate concentration of 30mM for all the four soils. With further increase in substrate concentration, minimal change in enzyme activity was observed. Characteristics of enzyme activities like maximum enzyme reaction velocity (Vmax) and Michaelis constant (Km) were determined using Michealis – Menten equation similar to those determined in homogenous system. The Km value range from 0.49mM to 0.60mM in Lineweaver - Burk Transformation and 0.50mM to 0.76mM in Hanes - Wolf Transformation and in case of Eadie - Hofstee Transformation the Km value range from 0.62mM to 0.78mM. Vertisols showed more km value than Alfisols. The Vmax value range from 8.1µg of NH4+ released g-1 soil h-1to 10 µgof NH4+ released g-1 soil h-1 in Lineweaver - Burk Transformation and 8.7(µg of NH4+ released g-1 soil h-1 to 10.3 (µg of NH4+ released g-1 soil h-1 in Hanes - Wolf Transformation and in case of Eadie - Hofstee Transformation the max range from 9.1(µg of NH4+ released g-1 soil h-1 to 10.5 (µg of NH4+ released g-1 soil h-1 . Vmax value range from 8.1 (µg of NH4+ released g-1 soil h-1to 9.7(µg of NH4+ released g-1 soil h-1 in Vertisols and 9.5 (µg of NH4+ released g-1 soil h-1 to 10.5 (µg of NH4+ released g-1 soil h-1 in Alfisols and Alfisols showed more Vmax value than Vertisols
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