Oyster mushroom constitute thirty per cent of total mushroom production and ranks third among the cultivated mushrooms grown widely in temperate, sub-tropical and tropical regions of the World. The present investigation was undertaken to evaluate the effect of nutritional medium medium, temperature and colour on mycelial growth behaviour of Hypsizygus ulmarius in vitro. The mycelium was dense and white in potato dextrose agar (PDA) medium and required least days for full growth (10.50 days) followed by Malt Extract Medium (11.25 days). Plates incubated in 25^°C required minimum time (10.25 days) to obtain full mycelial growth followed by 30^°C temperature (11.25 days). Out of different colour polythene wrapping, black colour polythene treatment gave the best result as it required the least days for spawn run (16 days) followed by blue colour (16.25 days) which provided uniform growth of the medium.

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