The experiment was carried out in the Department of Horticulture (Vegetable and Floriculture), Bihar Agricultural College, Sabour to study the role of bio fertilizers and chemical fertilizers on onion production. The experiment was laid out in a factorial randomized block design, replicated thrice with onion variety Agrifound Light Red. The experiment was framed with bio fertilizers viz., M₀- 0, M₁- Azotobacter, M₂-Azospirillum, M₃- PSB, M₄-VAM and four levels of chemical fertilizers, (F₁-N₈₀ P₄₀ K₆₀, F₂-N₁₀₀P₆₀K₈₀, F₃-N₁₂₀P₈₀K_100, F₄-N₁₄₀P₁₀₀K₁₂₀ having 20 treatment combinations. The results of the experiment showed that the fertility level F₃-N₁₂₀P₈₀K₁₀₀ with inoculation of M₂- Azospirillum i.e., F₃M₂ the maximum average bulb weight (77.53 g), diameter of bulb (6.17 cm), bulb length (5.97 cm), bulb yield (438.56 q/ha) was recorded F₃M₂ treatment combination, and treatment combination F₁M_O shows lowest value. However, inoculation of Azospirillum with soil application at the fertility level of F₃-N₁₂₀P₈₀K₁₀₀ was the most effective combination for higher net return and B: C ratio (4.70).

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