National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
In the current study prevalence and characterization of extended spectrum beta-lactamase(ESBL) production of Escherichia coli was detected from broilers of West Bengal, India. A total of 248 cloacal swabs samples were examined for E.coli by standard bacteriological techniques. E.coli isolates were serotyped and also screened for extended spectrum beta-lactamase by double disc diffusion assay and by PCR detection of blaCTX-M, blaSHV, and blaTEM genes. The antimicrobial sensitivity profile of the isolated E.coli was demonstrated against 13 antimicrobial agents. One hundred thirty one (52.82%) E.coli were identified and the isolates belonged to 12 different serogroups viz. O2, O20, O35, O83, O87, O88, O116, O117, O119, O128, O135, O141 and untyped isolates. Thirty seven (28.24%) of E.coli were detected as ESBL-positive by double disc diffusion assay. Among the studied genes, blaCTX-M gene was detected in 32 (24.43%), blaSHV gene in 3 (2.29 %) isolates and blaTEM gene in 2 (1.53%) isolates. Highest antimicrobial susceptibility of E.coli isolates was observed to chloramphenicol (69.36 %) and levofloxacin (62.9 %). Highest degree of resistance was observed to cefepime (95.12%) followed by nalidixic acid (88.88%), co-trimoxazole (83.33%), ampicillin (79.03%), cefotaxime (78.46%), tetracycline (72.58%) and ciprofloxacin (62.90%). The present study revealed that broilers were reserviors of ESBL producing E.coli and resistant to many classes of antimicrobials.
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