National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Papaya is a nutraceutical fruit crop grown among tropical and sub-tropical regions of the world. The propagation through seeds results in the production of non-true-type planting materials due to segregation of off-springs at second filial generation leading to inherent heterozygosity. The alternative strategy to this is in-vitro propagation, but the main hurdles comes under this method is failure to culture establishment. Thus, searching for an effective sterilization is always needed. Therefore, present study was intended to evaluate the effect of various sterilant on culture establishment. The lateral buds were collected as explant and treated with 0.1% Bavistin, 0.1% HgCl2, 4% NaOCl and 70% EtOH for different time interval. The higher survival percent and uniform growth pattern was observed with 0.1% Bavistin for 10 min (38.7), 0.1% HgCl2 for 2 min (40.5), 4% NaOCl (39) and 70% EtOH for 1 min (37.8), whereas combination of these sterilant showed 46.3% shoot survival with uniform growth.
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