National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Serum gonadotropin @50 and 100IU/ml, estrodiol-17β @0.5 and 1μg/ml, EBS @5 and 10% and BFF @5 and 10% supplementation Iis done on cleavage rate of buffalo oocytes for the comparison of efficacy of hormones. Ovaries were collected from buffaloes slaughtered at local abattoir. Oocytes were aspirated and good and excellent quality oocytes were matured in basic maturation media TCM199 supplementing above mentioned supplements separately. The recovery rate of culturable oocytes was 85.33%. The highest cleavage rate was documented with supplementation of 10% EBS and the lowest was observed with 10% BFF. The cleavage rate was significantly (p≤0.05) higher in the group supplemented with 5%EBS and 10% EBS as compared to all other groups of the experiment. It can be concluded that the supplementation of EBS to IVM medium has positive effect on cleavage rate.
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