National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Humic acids (HA) are widely used in agriculture because of their capability as chelator, organic matter, and soil improver and growth promoter. However, their isolation differs in terms of origin or source because of wetting and solubilisation factors. This study was conducted to determine extraction period for HA from organic sources. Standard procedures were used to extract HA from pressmud (PM) and vermicompost (VC) at different extraction periods of 4, 8, 12, 16, 20 and 24 hours and fractionation periods (3, 6, 9, 12, 15, 18, 21& 24 hrs). At the end of each extraction period, samples were centrifuged, followed by fractionation and purification. HA yield at different extraction periods was determined using standard procedures. The relationship between extraction period and HA yield was quadratic. Extraction and fraction period for PM & VC was 24 hours and purification (done in 1 h) using distilled water was effective in removing impurities. HA from PM and VC can be extracted and fractionated in 24 hours and purification can be done in one hour.
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