Studies throughout the world document that the nosocomial infections are a major cause of morbidity and mortality. Many factors contribute to the frequency of nosocomial infections like immunocompromised state of patients, invasive examinations and treatments, patient care practices and hospital environment which facilitate transmission of microbes among patients. Treatment is becoming difficult due to inappropriate use of antibiotics and hence resistance develops. Knowledge on antibiotic resistance pattern is essential for appropriate therapy. Objectives of the study are to evaluate the bacteriological profile causing Blood Stream and Urinary Tract Infections in a medical Intensive Care Unit. 2. To analyse the antibiotic susceptibility tests and hence to arrive the Antimicrobial resistance patterns from ICU patients. Blood samples and urinary samples from adult patients of both sexes admitted to ICU, for a period of six months, acquiring signs of sepsis will be analysed by blood culture done in BHI broth and MSU sample of urine collected and process in CLED media. Culture positives identified by growth in the selective media, confirmed by Biochemical tests and Antibiotic susceptibility tests done in Muller Hilton Agar by Kirby Bauer’s disc diffusion method. Hence Antimicrobial resistance profiles of bacterial strains were analysed. Total of 43 BSI and 296 urinary positive samples were obtained from Medical ICU over a period of six months in Apr- Sep 2019.The most prevalent BSI pathogens were Klebsiella pneumoniae, Staphylococcus aureus and CoNS Escherichia coli and Klebsiella spp. being the prevalent pathogens from urinary tract samples. The antimicrobial resistance profile from ICU patients were analysed for generating Antibiogram in a Tertiary care hospital. Vancomycin, Merapenam, Imipenam, Linizolid, Cefiperazone /Salbactum are most effective antibiotics against Gram positive bacteria. Amikacin, Gentamycin, Piperacillin/ Tazobactum and Nitrofurantoin are effective against nosocomial UTI infection caused by Gram negative bacteria. This study also guides to formulate Antibiotic policy which helps the Clinician to choose empirical therapy and switch over to for Escalation and Cycling of Antibiotics which favours Infection control methods.

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