Expression of RelA, IKBα and IKKβ Genes in peste des petits Ruminants Virus Infected Cells

Siddharth Gautam; Amol Gurav; Muthu Sankar; Nitish S. Kharayat; A. P. Madhusoodan; A. Rialch; D. Muthuchelvan; Chitra Joshi; Anil K. Sharma

doi:https://doi.org/10.20546/ijcmas.2020.901.192

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Original Research Articles Volume : 9, Issue:1, January, 2020

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Int.J.Curr.Microbiol.App.Sci.2020.9(1): 1730-1735

DOI: https://doi.org/10.20546/ijcmas.2020.901.192

Expression of RelA, IKBα and IKKβ Genes in peste des petits
Ruminants Virus Infected Cells

Siddharth Gautam^1*, Amol Gurav¹, Muthu Sankar¹, Nitish S. Kharayat¹, A. P. Madhusoodan¹, A. Rialch¹, D. Muthuchelvan², Chitra Joshi³ and Anil K. Sharma⁴

¹Division of Temperate Animal Husbandry; ²Division of Virology, ICAR-Indian Veterinary Research Institute, Mukteshwar, Nainital, Uttarakhand, India
³Animal Husbandry Department, Uttarakhand, India
⁴Division of Pathology, ICAR- Indian Veterinary Research Institute, Izatnagar,Bareilly, U.P., India

^*Corresponding author

Abstract:

Peste des petits ruminants (PPRV) is a highly contagious transboundary viral disease of small ruminants with widespread distribution and poorly characterized molecular pathogenesis. The present study aimed at delineating the role of NF-kB signalling pathway during PPRV infection in the in-vitro system. For this the gene expression of three major regulatory proteins of NF-kB pathway viz. IKBα, IKKβ and RelA were analysed in the PPRV infected and mock infected Vero cells as a function of time using SYBR-green based one-step RT-qPCR. The results genes revealed downregulation of IKBα gene and upregulation of RelA and IKKβ genes in the PPRV infected group as against mock infected during initial hours of infection.

Keywords: RelA, IKBα and IKKβ, peste des petits, RT-qPCR

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Siddharth Gautam, Amol Gurav, Muthu Sankar, Nitish S. Kharayat, A. P. Madhusoodan, A. Rialch, D. Muthuchelvan, Chitra Joshi and Anil K. Sharma. 2020. Expression of RelA, IKBα and IKKβ Genes in peste des petits Ruminants Virus Infected Cells.Int.J.Curr.Microbiol.App.Sci. 9(1): 1730-1735. doi: https://doi.org/10.20546/ijcmas.2020.901.192

Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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