National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Gram-negative bacilli (GNB) are most frequently isolated bacteria from ascitic fluid aspirate of patients with Spontaneous bacterial peritonitis (SBP). Aetiology of SBP has been changing in recent years with increased involvement of multidrug resistant (MDR) bacteria as a result of production of various β-lactamases. The present study was done for detection of different β-lactamases and their co-existence by noveldisc placement method among GNB isolates from patients with SBP. Disc panel containing ceftazidime (CA), ceftazidime/tazobactam (CA/TZ), cefepime (CFP), ceftazidime/ tazobactam/ cloxacillin (CA/TZ/CLOX), imipenem (IMP) and Mercaptopropionic acid (MPA) was used. Pureextended spectrum β-lactamase (ESBL) production was found in 18 of 42 isolates (42.86%). Maximum ESBL positive isolates were found in E.coli (13 out of 28 isolates).Pure AmpC production was found in 6 of 42 isolates (14.28%).Metallo-β-lactamases (MBL) production was found in only 2 of 42 isolates (4.76%). Co-production of ESBL+ AmpC was seen in 3 of 42 isolates (7.145). All ESBL positive isolates showed multidrug resistance pattern. Detection of β-lactamase producing isolates will provide knowledge regarding resistance pattern of bacterial strains in a particular geographical area. This will further help promote appropriate and judicious use of antibiotics.
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