National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Metallo-β-lactamases (MβLs) production in gram negative bacilli is an emerging health problem worldwide. We aimed to determine the occurrence of MβLs among gram negative bacilli (GNB) by phenotypic and genotypic methods in Mansoura University hospital, Egypt. We conducted a prospective study on 107 GNB isolates recovered from different clinical specimens collected from different patients. Species identification and antibiotic susceptibility profile was determined by MicroScan Wa1kAway system. GNB which showed resistance to imipenem and/or meropenem were screened for MBL production by double-disk synergy test (DDST). Phenotypically detected MBLs isolates were further studied for the presence of three genes; IMP, GIM and VIM by multiplex PCR. Out of 107 GNB isolates, 62 (57.9%) strains showed resistance to imipenem and/or meropenem. DDST detected MBLs in 46.8% of carbapenem resistant isolates.65.5% of phenotypically detected MβLs isolates were carrying genes for MβLs production. IMP was the most frequent detected gene (36.8%) followed by VIM, and GIM genes (31.6% for each). MβLs production were more frequent in E.coli, Pseudomonas species, Acintobacter species (26.3% for each) followed by Klebsiella species (15.8%) and Moraxella species (5.3%). Our findings showed production of MβLs in a considerable number of GNB which is alarming and need continuous surveillance to detect such resistant strains. DDST is simple method and could be routinely applied for early detection ofthis resistance to establish the proper antibiotic therapy and infection control measures to combat their spread. Future studies are needed for extensive molecular characterization of MβLsamong GNB in our locality.
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