National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Environmental contamination by toxic heavy metals is causing a serious problem through the worldwide due to their incremented accumulation in food chain and continued persistence ecosystem. Sixty-six isolates of actinomycetes were isolated from different drains in Egypt. Eleven isolates which removed studied heavy metals (Cu2+, Pb2+ and Cd2+) singly above 70% were selected to remove metals from ternary mixtures. The biosorption by dead biomass for all isolates was higher than that by living biomass. The highest removal was recorded by Kw42 isolate. It has removed 81.7%, 88.6% and 69.2% from Cu2+, Pb2+ and Cd2+, respectively. The 16S rRNA analyses and phylogenetic data of Kw42 concluded that Kw42 is a member of streptomyces genus and itwas deposited in the Gen Bank Database under accession number MK020765. The biosorption by the dead biomass of Streptomyces cyaneus Kw42 under the optimized conditions (pH-8 at 40ï‚°C for 3 h. with 0.3% biosorbent dosage) was found to be: Pb2+ (83.4%) > Cu2+ (74.5%) >Cd2+ (68.4%). By Electronic Microscope investigation the surface of dead biomass of Kw42 became smoother after binding with metal ions. Also, the Energy Dispersive X- raycharts after biosorption had copper peak at 8 Kev, lead peak at 2.8 Kev and cadmium peak at 3.1 Kev. Treatment of real wastewater by dead biomass of Streptomyces sp. Kw42 yielded complete bio-removal for all studied heavy metal ions after 120 min.
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