National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Charcoal rot caused by Macrophomina phaseolina is a serious and destructive disease in maize. In the present study, the biocontrol agents Trichoderma virens TRI 37 and Bacillus amyloloiquefaciens (VB7) were screened against M. phaseolina under in vitro, in which T. virens TRI 37 effectively inhibited the mycelial growth of pathogen to about 61.11% followed by B. amyloliquefaciens produced zone of inhibition and percent inhibition of mycelia growth with2.3 cm and 48.44% respectively. Different antimicrobial compounds produced by effective antagonists were analyzed. Agar well diffusion assay with the secondary metabolites of T. virens TRI37 and B. amyloliquefaciens (VB7) isolates were screened against M. phaseolina under in vitro in which B. amyloliquefaciens (VB7) produced zone of inhibition and percent inhibition of mycelial growth about 1.5cm and 87.11% followed by T. virens TRI 37 produced zone of inhibition and percent inhibition of mycelial growth about 1.16cm and 76% respectively. The fungal antagonists T. virens TRI 37 produced microbial non-volatile organic compounds (MNVOC) such as alanine-beta-naphthylamide, pyrazine, propanoic acid, octadecatrienoic acid, glutamine, hexadecenoic acid and decane. Similarly, the bacterial antagonists produced the microbial non-volatile organic compounds such as stigmasterol, nonadecane, cyclopropanol, piperazinedione, docosene, galactopyranoside, oleanane and ditertbutylphenol. Maize seeds were treated with fungal (108 conidia per ml) and bacterial antagonists (108 cells per ml) and sown under in vitro to assess the growth parameters of maize seedlings. The combination of T. virens TRI 37 and B. amyloliquefaciens (VB7) effectively increased the shoot length (17 cm), root length (22 cm) and stem girth (4 cm) followed by B. amyloliquefaciens (VB7) shoot length (15.5 cm), root length (17 cm) and stem girth (5.5 cm) as against control with shoot length (13 cm), root length (16 cm) and stem girth (2 cm).
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