National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
In a trial to search for a new antibiotic producer a terrestrial actinomycete was isolated from AL-alameen soil which proved to be a potential candidate for the production of active metabolites against E. coli 25922 and Staphylococcus aureus 25923. It was identified phenotypicallyas Streptomyces sp. The optimum activity was obtained when the organism was grown on glycerol as a sole carbon source at 30°C and pH 7.0 for 192 h. The highest metabolic activity noticed intracellulary and it was approved with MIC values. In a trial to test the combined activity of the extract and some antibiotics; synergistic action was noticed with ceftriaxone. Further investigations were applied to the extract for its active components using TLC and GC-MS which indicated that Pentadecanoic acid, 14-methyl-, methyl ester; Hexadecanoic acid, methyl ester and 2,4-bis(1,1-dimethylethyl)- Phenol were the potent active components. Toxicity test will be evaluated for the active components as well as the crude extract.
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