National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Bacteria resist antibiotic action through several mechanisms, including target al., teration, drug inactivation, decreased permeability, and increased efflux. The aim of the study is detection of EP mediated MDR phenotypes in E. coli by using EB agar Cartwheel method. The agar-based method is used to evaluate as many as twelve bacterial strains per plate and has been termed the EB-agar cartwheel method. E. coli strains were grown in 5 ml peptone water until they reached an optical density (OD) 0.6 at 600 nm, then OD of the cultures was adjusted to 0.5 of a McFarland standard. EB concentrations starting from 0, 0.5, 1, 1.5, 2 & 2.5 mg/l were prepared in distilled water on the same day of the experiment from stock solution (50 mg/ml, stored at 4˚C) and protected from light. The evaluation of efflux-pump activity was done on70 MDR E. coli strains isolated from urine specimens of clinically suspected UTI patients of age 18‑80 years. The prevalence of active efflux was observed in 60% (42/70) MDR E. coli strains. The high prevalence of an active efflux system in the present study in MDR E. coli isolates indicate that efflux activity-based antibiotic resistance is one of the mechanism of drug resistance prevalent in MDR E. coli isolates.
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