National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The Staphylococcus aureus is a versatile opportunistic pathogen affecting both human and animals. The Genotyping of S. aureus isolates helps to understand the pathogen’s dissemination and epidemiological clusters, Hence, the present study designed to study the genotyping Staphylococcus aureus with RS-PCR from Chicken meat in Chennai City. In the present study, ribosomal spacer PCR (RS-PCR) was found to be showing polymorphism with varying band size and on software based dendrogram analysis. The dendrogram obtained upon analysis found that 3 main clusters and named as Cluster A, Cluster B and Cluster C. The similarity co-efficient at 0.05 they were characterised as 23 ribotypes. The most frequent ribotype belong to Cluster C and ribotype no 23. Based on Cluster analysis in our study revealed that may be single evolutionary originated epidemiological similar S. aureus strain is circulating and its clustered with transmitted in chicken meat though out the city thou meat was not collected from the same farm or site. The PCR ribotyping is a rapid inexpensive technique that is highly reproducible and almost as discriminatory as other typing methods for S. aureus isolates and should be useful in the local investigation and control of outbreaks.
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