National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
An experiment was carried out at Department of Plantation Crops and Spices, College of Horticulture, Vellanikkara with an objective to develop a protocol for in vitro regeneration and also to determine the variation in morphogenesis and regeneration among elite cocoa clones. The experiment was done in two factorial CRD using five cocoa varieties, CCRP 2, CCRP 6, CCRP 8, CCRP 15 and Scavina. Single nodded cuttings of 2-3 cm collected from the budded plants maintained in polyhouse were used as explants. The best surface sterilization of explants was achieved using Mancozeb 0.2 per cent, Streptocycline 0.1 per cent, Ethyl Alcohol 70 per cent and Mercuric Chloride 0.1 per cent. Axillary bud sprouting was induced in basal Woody Plant Media (WPM) fortified with 2-Isopentinyl Adenine (2ip) and Indole-3-Acetic Acid (IAA). The period of single shoot induction from leaf axils was lowest in variety CCRP 15 in media WPM+2ip 1ppm+IAA 0.02ppm. Among the varieties, highest multiple shoot induction percentage was recorded in variety Scavina.
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