National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Entero-parasites protozoan of the genera Cryptosporidium and Giardia have emerged over the past decades as major waterborne pathogens with an increasing number of outbreaks reaching over one hundred and sixty. In line with the national goals of providing safe drinking water supplies, this research was designed. We aimed to estimate the prevalence of Cryptosporidium parvum and Giardia intestinalis infections in surface water plants from ShebinAl-Kom and Tala, Menoufia Governorate and to evaluate routine water purification methods used for parasitic elimination. The following methods were used for assessment of samples; mechanical filtration, staining techniques and qPCR assay for detecting DNA of (oo) cysts n water samples.Water samples collected over one year (four seasons) were 87 samples. Microscopic examination (our golden test) revealed, 49 samples positive for cryptosporidium, fourteen positive samples for Giardia and four samples were positive for both of them whereas, qPCR revealed only eight positive samples for Giardia and ten positive samples for cryptosporidium. A statistically significant result concerning seasonal variation was documented where water contamination crested in Summer (p < 0.05). The intensity of infection decreased significantly after water treatment in both Shebin and Tala water stations (P= 0.01).From the present survey, we found that the prevalence of giardiasis and cryptosporidiosis accounted for 29.8% and 47.1% in Shebin Al-Kom and Tala respectively. The parasitic cyst wall structure rendered diagnosis by qPCR. Genotyping of water samples is recommended for sourcing of infection which may be accused of water-borne outbreaks.
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