National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present study was undertaken to identify the presence of anti-leptospiral antibodies in the bovine serum samples using two serological tests, a genus specific indirect ELISA and a serovar specific microagglutination test (MAT). A total of 250 serum samples from bovine leptospirosis suspected cases were sampled in and around Pondicherry (Southern India). The anit-leptospiral antibodies were detected by indirect ELISA using outer membrane proteins (OMP’s) of Leptospira interrogans serovar pomona as antigen and compared with MAT using six infecting serovars (hardjo, icterohaemorrhagiae, grippotyphosa, pomona, canicola and australis) as antigen. Statistical analysis of the data was performed using chi-square test. Out of 250 sera samples, 91 (36.4 %) and 62 (24.8%) were positive by indirect ELISA and MAT respectively. The indirect ELISA employed in this study revealed a very high sensitivity of 98.38 % in comparison to 67.03 % in MAT. Chi –square analysis revealed a significant difference (i.e. P<0.001) between the two serological tests. This study revealed that indirect ELISA was found to be highly sensitive, rapid and easy to perform for detection of bovine anti-leptospiral antibodies in comparison with MAT.
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