National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
An experiment was conducted using seeds of two mungbean verities viz., WGG-42 and LGG-460 treated with various doses/ concentrations of gamma rays (200, 300, 400, 500 and 600 Gy) and ethyl methane sulphonate (0.2%, 0.3%, 0.4%, 0.5% and 0.6%) and sodium azide (1 mM, 2 mM and 3 mM) to find the LD50 dose by using probit analysis in M1 generation. LD50 dose is important to understand the sensitivity of various genotypes to the critical dose of mutagens creating 50 percent mortality. LD50 value for gamma rays in WGG-42 and LGG-460 was 565.63 Gy and 448.33 Gy, respectively. Similarly, LD50 value for EMS in WGG-42 and LGG-460 was 0.46% and 0.42%, respectively. Whereas, LD50 value for SA in WGG-42 and LGG-460 was 2.72 mM and 2.01 mM, respectively. The effect of mutagens on biological parameters like germination (%), shoot length and root length studied under laboratory conditions in M1 generation. The germination (%), shoot length and root length decreased progressively with increasing doses of gamma rays, EMS and SA in both the varieties of mungbean. There is a difference of LD50 values of mutagens among different varieties which indicated that there is a varied sensitivity for different mutagens on WGG-42 and LGG-460. The variety LGG-460 is found more sensitive to various mutagens when compared to WGG-42.
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