National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Escherichia coli and Salmonella are considerably responsible for causing gastrointestinal disorders. Emergence of Extended spectrum beta lactamase (ESBL) production among the above organisms is a great matter of concern. Hence, the present study is envisaged to know the distribution status of ESBL producing E. coli and Salmonella spp. from raw milk samples. A total of 50 raw milk samples was collected and were analyzed for the E. coli and Salmonella through conventional culture method and confirmation by PCR using uidA gene (E. coli) and invA gene (Salmonella). The occurrence of E. coli and Salmonella spp in raw milk was found to be 60 per cent and 18 per cent, respectively Further, characterization of isolates for phenotypic ESBL production revealed that 21 E. coli (70%) and 7 Salmonella (77.77 %) as phenotypic ESBL producers and were resistant to third generation cephalosporins. blaTEM, blaCTX-M and blaSHV gene was present in 56.66 per cent, 40 per cent and 26.66 per cent of the E. coli isolates and the occurrence of blaSHV, blaCTX-M and blaTEM gene in Salmonella isolates was 55. 5 percent, 44.4 per cent and 11.1 per cent, respectively. Thus the present study envisaged the need of hygienic milk production and proper antibiotic usage to prevent further spillover of resistant pathogenic organisms among public and environment.
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