The aimed of the study is screening of antibiotic producing Streptomyces isolates. Thirty soil samples, collected from different areas in the city of Baghdad, were screened for Streptomyces effectiveness as a source for active antibacterial, 26 (86.6%) samples were suspected to contain Streptomyces, out of them, 24 (80%) isolates were obtained with different morphological characteristics. Suspected Actinomycetes colonies were sub-cultured in ISP2 agar media carefully to obtain a pure isolate which was characterized as colored in aerial and substrate mycelium, dried, rough\ smooth, with an irregular/regular margin; generally convex colony. The isolates were identified as Streptomyces sp. based on their morphological, physiological and biochemical characteristics. Most Streptomyces isolates were screened for their antibacterial activity against Escherichia coli and Staphylococcus aureus on malt extract yeast extract agar medium (ISP2) using a cross-streak technique.

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