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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 8, Issue:11, November, 2019

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(11): 2308-2322
DOI: https://doi.org/10.20546/ijcmas.2019.811.268


Identification and Molecular Characterization of Groundnut Bud Necrosis Virus (GBNV) Infecting Chilli (Capsicum annuum L.) in Andhra Pradesh, India
Bhagavathi Devi*, M. Krishna Reddy, B. Padmodaya, V.L.N. Reddy and V. Srilatha
Department of Plant Pathology, S.V Agriculture College, Tirupati, Andhra Pradesh, India
*Corresponding author
Abstract:

Groundnut Bud Necrosis Virus (GBNV) (family Bunyaviridae, genus Tospovirus) is an emerging plant viral disease. The GBNV was a very broad host range infecting many economically important crops throughout in India. So, the aim of this study is identification of GBNV infecting chilli to know the genetic diversity. The plants showing chlorotic and nectrotic rings and apical bud necrosis in young leaves of chilli plants were collected from different locations in Andhra Pradesh. Total RNA was isolated from the positive ELISA samples and amplified with GBNV coat protein gene specific primers. GBNV chilli isolates shares a tripartite ssRNA genome structure and organization with other GBNV isolates, and more generally, other tospoviruses. The genome consists of L RNA (8911 nt), M RNA (4816nt) and S RNA (3059nt) segments.


Keywords: GBNV, RNA, Genome, Nectrotic rings, Grown forits pungency, Color

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How to cite this article:

Bhagavathi Devi, D., M. Krishna Reddy, B. Padmodaya, V.L.N. Reddy and Srilatha, V. 2019. Identification and Molecular Characterization of Groundnut Bud Necrosis Virus (GBNV) Infecting Chilli (Capsicum annuum L.) in Andhra Pradesh, India.Int.J.Curr.Microbiol.App.Sci. 8(11): 2308-2322. doi: https://doi.org/10.20546/ijcmas.2019.811.268
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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