National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
In the present study, total 100 cloacal/intestinal swabs from poultry were collected from the retail chicken meat shop from the city of Junagadh, Gujarat. Of this, total 80 (80%) isolates were identified morphologically and biochemically as E. coli. The antibiotic resistance patterns of all these isolates were studied using disc diffusion method and showed 97.5% isolates were resistant to ceftriaxone/ salbactum followed by cefotaxime/clavulanate (96.25%), cefoxitin/cloxacillin (92.5%), cefixime (92.5%) and ceftizoxime (92.5%).Total 13 (16.25%) isolates were found phenotypically ESBL producing E. coli. Of these, 4 isolates shown difference of ≥5 mm zone of inhibition between cefotaxime and cefotaxime + clavulanate discs, 10 isolates shown difference of ≥5 mm zone of inhibition between ceftriaxone and ceftriaxone + salbactum discs and 3 isolates shown difference of ≥5 mm zone of inhibition between ceftazidime and ceftazidime + clavulanate discs and 28 isolates were found positive for the presence of blaTEM gene (445 bp) using molecular based detection, whereas none of the isolates shown desired amplification to blaSHV and blaCTX-M genes, indicating blaTEM gene is the most abundant ESBLs type prevails in this region.
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