National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Theileriosis is a tick borne haemoprotozoan disease caused by Theileria annulata and Theileria orientalis, mainly. The disease is known to be highly fatal and is also responsible for heavy economical loses in dairy industry. In the present study, blood samples were collected from the buffaloes suspected for theileriosis. A total of 50 blood samples from jugular vein of buffaloes were collected in EDTA tube and a drop of fresh blood was also taken on FTA card for DNA isolation. For Polymerase chain reaction, the primer sequences were used as per Kakati et al., and Kolte et al., The PCR assay employs primers specific for the gene encoding 30kDa major merozoite surface antigen of Theileria annulata and major piroplasm surface protein gene of Theileria orientalis. Out of total 50 blood samples, 13 were positive for Theileria annulata by both whole blood PCR and FTA card PCR whereas for Theileria orientalis, 23 were positive by whole blood PCR and 27 were found positive by FTA card PCR. It was found that diagnosis of Theileriosis by using FTA card is highly recommended as it is sensitive, less laborious, less chances of DNA degradation, easy and quickest method of diagnosis as compared to Whole blood.
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