National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present investigation was carried out at Tissue Culture Laboratory located in Sugarcane Research Station, Cuddalore on in vitro shoot regeneration of sugarcane apical meristem during the period of 2018-2019. The regeneration of sugarcane variety CoC25 with supplementation of cytokinin 0.1mg/l BAP, 0.015mg/lkin, 0.5mg/lGA3 was used in sugarcane on defined MS media for shoot induction. The concentration of 0.25mg/lBAP, 0.5mg/lkin,1.0mg/lGA3, 0.5mg/l NAA involved in shoot regeneration in variety CoC25 showed the highest shoot length, number of tillers and chlorophyll content. The concentration of ½ MS, 4mg/l NAA and 40g/l sucrose as only auxin involved in root initiation showed the highest number of roots and root length. The sugarcane plantlets were acclimatized in greenhouse before planting in the field.
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