National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Riemerella anatipestifer, a pathogen causing infectious serositis and septicaemia in ducks, turkey, geese, pheasants, swans, poultry and also reported in pigs, which belongs to family Flavobacteriaceae. Twenty one serotypes have been reported so far by serum agglutination test which is found to be labour intensive and time consuming. In order to find out the better method to differentiate the isolates, PCR-RFLP and REP-PCR has been compared. The 20 isolates of R. anatipestifer were subjected to 16S rRNA PCR and subjected to restriction digestion with Hae III and the banding pattern was observed for differentiation. On amplifying the repetitive extragenic palindromic sequences of R. anatipestifer, the electrophoretic patterns of PCR products obtained were considered for the classification. In both methods, the differences in banding pattern were analysed using PyElph 4.1 software for dendrogram construction. Both methods differentiated the isolates into three groups whereas REP-PCR was found to be the most efficient method owing to its easiness and repeatability. Hence, REP-PCR could be used for epidemiological investigation and characterisation of R.anatipestifer isolates.
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