National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Diagnosis of disease is the backbone of control and treatment in veterinary field. In addition to the antemortem and post mortem methods, currently several laboratory-based tools and technique are also being used for early diagnosis. Since few decades, polymerase chain reaction (PCR) has emerged as the most preferred molecular diagnostic technique for disease diagnosis due to its high specificity. But it only detects the presence of the target nucleic acid in the sample without quantifying the same. Additionally, the detection of amplified DNA requires one extra step of gel electrophoresis followed by visualization under ultraviolet rays which involves radiation hazards. Hence, a more sophisticated technique called real time polymerase chain reaction (PCR) has been discovered for developing rapid assay for the diagnosis of many diseases. Along with the detection of particular nucleotide sequence, quantification of the latter can also be performed using this assay. Real time PCR was either of two specific chemistry: Nonspecific DNA binding dye or specific hybridization probe. The fluorescence generated from either of the above during the assay is directly proportional to the quantity of target being amplified at the real time. Although field application of real time PCR is infrequent, nevertheless its rapidity, high sensitivity & specificity and less contamination risk may lead to its enhanced application in screening and epidemiological study in the veterinary field in recent future. In this review we attempted to brief about the chemistries of real time PCR and its application in diagnosis of different veterinary diseases worldwide.
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