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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 8, Issue:10, October, 2019

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(10): 1812-1818
DOI: https://doi.org/10.20546/ijcmas.2019.810.210


Pretreatment of Rice Straw using Deep Eutectic Solvent and Saccharification of Pretreated Residue by Crude Cellulase Enzyme
Poonam Maan* and R. S. Sengar
Department of Agriculture Biotechnology, College of Agriculture, SardarVallabhbhai Patel University of Agriculture and Technology, Meerut-250110, India
*Corresponding author
Abstract:

The present study demonstrated the pretreatment of rice straw using deep eutectic solvent (DES), choline chloride:urea and its comparison with acid and alkaline pretreatment. At a solid loading of 10%, choline chloride (ChCl):urea (1:2) was very effective in lignin extraction from rice straw at 45 °C. It is showed that nearly 40% of lignin was separated in a single step. However, enzymatic hydrolysis of pretreated rice straw with crude cellulase enzyme produced from C.cinereaRM-1 showed the reducing sugar yield of 385±8 mg/g with a saccharification efficiency of 31.5±2.5 % in 48 h at 10 % solids loading.


Keywords: Woody biomass, Agricultural residues, Rice straw, Lignin extraction, Fossil fuel

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How to cite this article:

Poonam Maan and Sengar, R. S. 2019. Pretreatment of Rice Straw using Deep Eutectic Solvent and Saccharification of Pretreated Residue by Crude Cellulase Enzyme.Int.J.Curr.Microbiol.App.Sci. 8(10): 1812-1818. doi: https://doi.org/10.20546/ijcmas.2019.810.210
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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