National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Research in plant biotechnology is playing a crucial role in the production and conservation of plant-based resources globally. The aim of the work was to establish favourable culture medium condition for regeneration and better growth of Tinospora cordifolia explant. Plant Micropropagation has also been used as a tool for the propagation of genetically manipulated superior clones. Our attempt was made to develop micropropagation as suitable condition for cloning of Tinospora cordifolia. The explants were cut into desirable size and inoculated into regeneration medium consisting of Murashige and Skoog basal combination and various combinations of hormones. The cultures were incubated at 16:8 hrs photoperiod and temperature of 27 + 2°. Nodal explants produced proliferation of shoots on the medium containing nodal explants produced proliferation of shoots on the medium containing BAP and IAA at different combinations. Results were obtained showing regeneration frequency after 14 and 21 days.
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