National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The objective of the present study was to identify protease producing bacteria isolated from food processing industries. Isolation of the organism was performed by serial dilution agar plate technique and initial screening of protease production was done using gelatine agar plates by flooded the plates with mercuric chloride solution (HgCl2-15g and 20 ml of 6.0 N HCl made up to 100 ml with distilled water). A total of 5 isolates were selected based on zone diameter in gelatine clear zone method and named as TKMFT8, TKMFT22, TKMFT25, TKMFT39 and TKMFT53. Initial identification of the selected isolates was carried out using cultural characterization, microscopic observation and Biomerieux VITEK 2 system identification based on biochemical reactions and identified as Staphylococcus sciuri (TKMFT8, TKMFT22, TKMFT25, and TKMFT39) and Achromobacter xylosoxidans (TKMFT53). Protease producing bacterial isolates can be used for the degradation proteinaceous waste material from food manufacturing units leading to recycling of food industry waste.
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