National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
A Pseudomonas aeruginosa TU isolated from diesel-contaminated site degraded 66% of an initial 10 mM naphthalene load after 48 h of incubation in a minimal-salts medium containing NH4Cl as nitrogen source whereas, the same bacterium, when incubated in the minimal-salts medium containing KNO3,degraded 10 and 20 mM initial naphthalene load within in 24 and 36 h respectively. The naphthalene enrichment medium containing NH4+ as nitrogen source slowed bacterial growth and compromised cell viability over 72 h while, naphthalene-minimal salts medium when supplemented by NO3-showed uniform and efficient cell growth. The bacterial degradation of naphthalene in the minimal-salts medium containing KNO3 resulted in transient accumulation of salicylate (0.11mM) and catechol (102 µM) after 12 and 24 h respectively whereas, in the medium containing NH4Cl, the naphthalene biodegradation caused decrease in the pH of culture medium from 7 to 3.8 and accumulation of 0.63 mM salicylate and 24 µM catechol as dead end products. Further, the cell-free extracts of the bacterium grown on naphthalene in the medium containing KNO3showed high activities of naphthalene-degrading enzyme as compared to that of the cell-free extracts of bacterium grown on naphthalene and NH4Cl.
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