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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 8, Issue:10, October, 2019

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(10): 352-360
DOI: https://doi.org/10.20546/ijcmas.2019.810.036


Prevalence of Salmonella in Bovine Fecal Samples, Slaughter House Samples and Environmental Samples
R. Nethra1, P. T. Ramesh2, B. M. Veeregowda3, C. Ansar Kamran1, A. Muralidhara4 and M. L. Sathyanarayana5
1Department of Veterinary Medicine, Veterinary College, Hebbal, Bengaluru, India
2Peripheral Veterinary Hospital, Yelahanka, Bengaluru, India
3IAH & VB, Bengaluru, India
4Dept. of VCC, Veterinary College, Hebbal, Bengaluru, India
5Department of Veterinary Pathology, Veterinary College, Hebbal, Bengaluru, India
*Corresponding author
Abstract:

Salmonella is widely distributed in bovine in different countries and is considered the most important related zoonotic diseases today which have a public health and economic importance. The present study was conducted to survey the prevalence of Salmonellosis in bovine fecal samples collected in ten different herds located at relatively different geographical areas, slaughter house samples and environmental samples by using standard microbiological and biochemical tests and confirm the isolated Salmonella by polymerase chain reaction targeting invA gene, specific at genus level. Six Salmonella isolates were recovered by screening 508 samples (388 bovine fecal samples from 10 different bovine herds, 57 lymphnode and gall bladder samples from slaughter house, 63 environmental samples from the bovine herds and Slaughter house) in the study. On an overall, the prevalence of Salmonella reported was 1.2% (6/508). The herd prevalence in the study was 20% (2 out of 10 herds). Whereas, the observed prevalence of Salmonella in bovine fecal samples, slaughter house samples and environmental samples were 1.04%, 1.75% and 1.6%, respectively. Polymerase chain reaction targeting invA gene confirmed the presence of Salmonella in the samples positive for Salmonella by culture method.


Keywords: Bovine, PCR, Prevalence, Salmonella, Slaughter house

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How to cite this article:

Nethra, R., P. T. Ramesh, B. M. Veeregowda, C. Ansar Kamran, A. Muralidhara and Sathyanarayana, M. L. 2019. Prevalence of Salmonella in Bovine Fecal Samples, Slaughter House Samples and Environmental Samples.Int.J.Curr.Microbiol.App.Sci. 8(10): 352-360. doi: https://doi.org/10.20546/ijcmas.2019.810.036
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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