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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 8, Issue:9, September, 2019

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(9): 2869-2874
DOI: https://doi.org/10.20546/ijcmas.2019.809.329


Isolation and Characterization of Indigenous Probiotic Bacteria from Silkworm (Bombyx mori L.) Gut
M. Saranya1*, S. V. Krishnamoorthy1, D. Balachandar2 and M. Tilak3
1Department of Sericulture, Forest College and Research Institute, Mettupalayam, India
2Department of Agrl. Microbiology, TNAU, Coimbatore, India
3Department of Forest products and utilization, Forest College and Research Institute, Mettupalayam, India
*Corresponding author
Abstract:

Lactic acid bacteria are described as heterogeneous group of regular, gram-positive, rod shaped, non-motile, non-spore forming bacteria with absence of catalase enzyme. Laboratory study was undertaken to isolate and characterize the lactic acid bacteria from silkworm gut in phenotype basis. A total of 21 silkworm gut samples were collected from silkworm breeds, Double hybrid {(CSR 6 X CSR 26) X (CSR 2 X CSR 27)} obtained from Chawki rearing center (CRC) at Annur, Coimbatore. The bacteria samples were isolated after growth on MRS agar medium and pure culture was obtained by sub-culturing on the same medium. Purity of each culture was confirmed by morphological investigation, Gram’s staining and further identification were done by lactic acid bacteria through specific biochemical tests. Finally eight bacterial isolates (SWGL 6, SWGL 7, SWGL 8, SWGL 9, SWGL 10, SWGL 11, SWGL 23 and SWGL 30) were identified as lactic acid bacteria group after morphological, cultural and biochemical characterization.


Keywords: Silkworm gut samples, culture and biochemical characteristics and lactic acid bacteria

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How to cite this article:

Saranya M., S. V. Krishnamoorthy, D. Balachandar and Tilak M. 2019. Isolation and Characterization of Indigenous Probiotic Bacteria from Silkworm (Bombyx mori L.) Gut.Int.J.Curr.Microbiol.App.Sci. 8(9): 2869-2874. doi: https://doi.org/10.20546/ijcmas.2019.809.329
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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